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Article In Press

August 2016

Volume 1 Issue 1

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Reseaarch Article

Is there an Effect on Liver and Renal Function Tests on Blood Storage With and Without Irradiation?

Kiran Dahiya*, Deepika Dalal, Monica Verma, Veena Singh Ghalaut, PK Sehgal, Rakesh Dhankhar

Blood for transfusion is stored using additives to preserve and to prevent coagulation and is pre-irradiated with gamma radiation to prevent graft versus host disease in the recipient. Biochemical storage lesions are still observed in this blood. This study was planned to observe the effect of storage on plasma urea, creatinine, uric acid, total protein, aspartate transaminase (AST), alanine transaminase (ALT) and lactate dehydrogenage (LDH) on 0, 3, 7, 14 and 21 days with and without prior irradiation.

 

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Research Article

Thermodynamic and Kinetic Properties of Lactate Dehydrogenase in Red Muscle of the Mesotherm Thunnus obesus

Chie Takahashi, Tatsufumi Goto, Mizuki Nakagawa, Shigeyoshi Nakamura, Shun-Ichi Kidokoro, Hideki Wakui, and Wataru Nunomura*

Although a number of studies on enzyme activities in ectotherms have clarified adaptation to environmental temperature, mesotherms have not been investigated in detail. Bigeye tuna Thunnus obesus is a cold-blooded animal; however, they are mesothermic because they have a unique thermoregulatory system that maintains body temperature at 21–26°C. We characterized the kinetic and thermodynamics of lactate dehydrogenase (LDH) in red muscle of T. obesus. Purified LDH from T. obesus red muscle (T. obesus LDH) migrated near the position of the M3H isozyme from bovine muscle on native polyacrylamide gel electrophoresis.

             

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